Prof.이규도

Dr. Gyudo Lee is an assistant professor of Biotechnology and Bioinformatics, Korea University. He received his B.S. and Ph.D. degrees from Department of Biomedical Engineering, Yonsei University in 2008 and 2014, respectively. He completed his postdoctoral training at Harvard University in 2017. He joined Korea University as a research professor of Biomedical Engineering. After research professor career, he has served on Korea University faculty in Sejong Campus since September 1st, 2019. Prof. Lee has been leading the Biomedical Nanoengineering Laboratory (BiNeLa) in the College of Science and Technology in Korea University. Main research interest of Prof. Lee focuses on the investigation of biomolecular interactions related to degenerative diseases using nanomaterials and nanotechnologies.

저는 고려대학교 생명정보공학과 이규도입니다. 2008년 및 2014년에 각각 연세대학교에서 학사 및 박사학위를 수여받았습니다. 1년동안 연세대학교에서 박사후연구원을 거친후 미국 Harvard 대학교에서 2년간 박사후연구원 과정을 수행하였습니다. 2017년부터 고려대학교 바이오의공학부 연구교수를 거쳐, 2019년 9월 1일부로 고려대학교 생명정보공학과에 부임하였고, 생체나노공학연구실(BiNeLa)을 이끌고 있습니다. 주된 연구분야는 나노소재와 나노기술을 융합하여 퇴행성질환과 관련된 생체분자의 거동특성을 연구하는 것으로, 궁극적으로 질병을 치료하거나 진단하는데 도움이 되는 나노기술을 개발하고자 합니다.

Contact Information

Biomedical Nanoengineering Laboratory (BiNeLa), College of Science & Technology, Korea University, Sejong 30019, Republic of Korea
■ Phone: 044-860-1413
■ E-mail: lkd0807@korea.ac.kr or leegyudo@gmail.com

Academic Career

■ 2019. Sep – Present: Assistant Professor, Department of Biotechnology and Bioinformatics, Korea University
■ 2017. Feb – 2019. Aug: Research Professor, School of Biomedical Engineering, Korea University
■ 2015. Feb – 2017. Jan: Postdoc Fellow, Molecular and Integrative Physiological Sciences (MIPS) Program, Harvard University
■ 2014. Mar – 2015. Jan: Postdoc Researcher, Department of Biomedical Engineering, Yonsei University

Education

■ 2008. Mar – 2014. Feb: Ph.D., Biomedical Engineering, Yonsei University
■ 2004. Mar – 2008. Feb: B.S.E., Biomedical Engineering, Yonsei University

Research Interests

■ Surface plasmonic drug-screening for Alzheimer’s diseases
■ Cell membrane-coating for molecular detection
■ Hydrogel scaffold-based controlled drug release for bone remodeling
■ Detection of toxic metal ion and nitric dioxide gas
■ AFM-based nanoindentation study of cells and tissues

Awards

■ 제2회 대한민국 바이오의약품 대상 공모전 한국바이오협회 회장상 (Nov 1st, 2019)
■ Korea University Grant (2017, 2018)
■ Brain Korea 21 full scholarship for graduate students (2008~2014)

■ 알츠하이머 약물 스크리닝 기술 개발

알츠하이머의 발생 기전은 아직 명확하게 밝혀지지 않았다. 그에 따라 알츠하이머를 진단하거나 치료하는 약물 개발은 답보상태이다. 현재까지 밝혀진 알츠하이머 발병 가설은 베타아밀로이드의 변성과 축적으로 인한 뇌조직의 괴사, 그리고 변성된 타우단백질의 신경세포내 축적, 그로 인한 세포 사멸이 주요 원인으로 꼽힌다. 현재 대부분의 알츠하이머 치료제는 질병의 진행을 예방하거나 지연시키는 작용에 초점을 맞추어 개발이 진행 중이다. 하지만 이미 발병한 환자의 경우에는 아밀로이드의 제거와 배출이 질병 치료에 있어서 필수적일 것이다. 본 연구실에서는 이미 형성된 변성 단백질들을 제거할 수 있는 약물 후보군을 빠르게 발굴하고 약효를 비교분석할 수 있게 해주는 초고속 알츠하이머 분해제 스크리닝 기술을 개발하고자 한다.

■ 세포막 코팅 기술을 활용한 바이오마커 센서 개발

인간의 몸을 구성하는 세포의 종류는 200가지가 넘는다. 각 세포 종류마다 세포의 막에 발현되는 막단백질의 종류와 농도는 매우 다양하고, 그에 따라 각 조직과 장기에서 적절한 기능이 이루어질 수 있다. 그 적절한 기능이란 세포의 표면에 발현된 막단백질의 기능으로 대변될 수 있을 것이다. 이러한 사실은 우리에게 한 가지 흥미로운 영감을 주게 된다. 그것은 바로, 만약 세포막을 추출하여 전극에 코팅할 수 있다면 우리는 특정 세포가 발현한 막단백질을 활용하여 분자센싱을 하거나 분자필터링을 할 수 있게 된다는 것이다. 본 연구실에서는 다양한 세포 종류별로 세포막을 추출 및 코팅하는 기술을 활용하여 막단백질의 기능에 대해서 심도있는 연구를 수행하고 궁극적으로 그 기능을 활용하여 다양한 바이오센싱 신기술을 구현하고자 한다.

Cover Article

Cover Article

2011 Chem Comm	2012 Angew Chemie	2012 J Mater Chem
2013 Nanoscale	2015 Anal Chem	2016 Nanoscale
2016 Macromol Res	2016 Anal Chem	2018 Nanoscale

Journal Publication

■ H. Lee, Y. Hong, D. Lee, S. Hwang, G. Lee, J. Yang, D.S. Yoon. Surface Potential Microscopy of Surfactant-Controlled Single Gold Nanoparticles. Nanotechnology. under review. 2019.Oct.30
■ W. Kim, H. Park, M. Kim, W. Lee, G. Lee*, J. Park. Eco-friendly and enhanced colorimetric detection of aluminum ions using pectin-rich apple extract-based gold nanoparticles. Nanotechnology. under review. 2019.Oct.7.
■ S. Jo, W. Lee, J. Park, W. Kim, W. Kim, G. Lee, H.J. Lee, J. Hong, J. Park. Localized surface plasmon resonance aptasensor for the highly sensitive direct detection of cortisol in human saliva. Sensors and Actuators B – Chemical. accepted for publication. 2019.Nov.16.
■ J. Park, W. Lee, G. Lee#, H. Choi, M. Kim, W. Kim, S. Na, J. Park. Electrochemical Amyloid-Based Biosensor for the Determination of Metal Ions. Journal of the Electrochemical Society. 166(15), B1497-B1505. 2019.

Prior to joining 고려대학교 생명정보공학과 (from the past to 2019.Aug.)

■ H. Choi, W. Lee, G. Lee, D.S. Yoon, S. Na. The Formation Mechanism of Segmented Ring-Shaped Aβ Oligomers and Protofibrils. ACS Chemical Neuroscience. 10(8), 3830-3838. 2019.
■ J. Park, W. Lee, I. Kim, M. Kim, S. Jo, W. Kim, H. Park, G. Lee, W. Choi, D.S. Yoon, J. Park. Ultrasensitive Detection of Fibrinogen Using Erythrocyte Membrane-Draped Electrochemical Impedance Biosensor. Sensors and Actuators B - Chemical. 293, 296-303. 2019. 다수 언론에 본 연구성과가 보도됨.
■ S. Jo, I. Kim, W. Lee, M. Kim, J. Park, G. Lee, D.S. Yoon, J. Park. Highly Sensitive and Wide-Range Nanoplasmonic Detection of Fibrinogen Using Erythrocyte Membrane-Blanketed Nanoparticles. Biosensors and Bioelectronics. 135, 216-223. 2019. 다수 언론에 본 연구성과가 보도됨.
■ I. Kim, D. Kwon, D. Lee, G. Lee*, D.S. Yoon. Permselective Glucose Sensing with GLUT1-Rich Cancer Cell Membranes. Biosensors and Bioelectronics. 135, 82-87. 2019. 다수 언론에 본 연구성과가 보도됨.
■ D. Lee, H. Lee, G. Lee, I. Kim, S.W. Lee, W. Kim, S.W. Lee, J.H. Lee, J. Park, D.S. Yoon. Extremely Sensitive and Wide-Range Silver Ion Detection via Assessing the Integrated Surface Potential of a DNA-Capped Gold Nanoparticle. Nanotechnology. 30(8), 085501. 2019.
■ W. Lee, Y. Choi, S.W. Lee, I. Kim, D. Lee, Y. Hong, G. Lee*, D.S. Yoon. Microwave-Induced Formation of Oligomeric Amyloid Aggregates. Nanotechnology. 29(34), 345604. 2018.
■ S.W. Lee, W. Lee, D. Lee, Y. Choi, W. Kim, J. Park, J.H. Lee, G. Lee*, D.S. Yoon. A Simple and Disposable Carbon Adhesive Tape-Based NO2 Gas Sensor. Sensors and Actuators B-Chemical. 266, 485-492. 2018.
■ G. Lee, W. Lee, S. Baik, Y.H. Kim, K. Eom, T. Kwon. Correlation Between the Hierarchical Structures and Nanomechanical Properties of Amyloid Fibrils. Nanotechnology. 29(29), 295701. 2018.
■ G. Lee, L. Atia, B. Lan, Y. Sharma, L. Nissim, M.-R. Wu, E. Pery, T.K. Lu, C.Y. Park, J.P. Butler, J.J. Fredberg. Contact Guidance and Collective Migration in the Advancing Epithelial Monolayer. Connective Tissue Research. 59 (4), 309-315. 2018.
■ D. Lee, G. Lee*, D.S. Yoon. Anti-Aβ Drug Candidates in Clinical Trials and Plasmonic Nanoparticle-Based Drug-Screen for Alzheimer’s Disease. Analyst. 143(10), 2204-2212. 2018.
■ I. Kim, D. Kwon, D. Lee, T.H. Lee, J.H. Lee, G. Lee*, D.S. Yoon. A Highly Permselective Electrochemical Glucose Sensor Using Red Blood Cell Membrane. Biosensors and Bioelectronics. 102, 617-623. 2018. 연합뉴스, 서울경제, 뉴스1 등 다수 언론에 본 연구성과가 보도됨.
■ D. Bang, T. Lee, J. Park, G. Lee, S. Haam, J. Park. Enhancement of Capturing Efficacy for Circulating Tumor Cells by Centrifugation. BioChip Journal. 12(1), 38-45. 2018.
■ W. Lee, S.W. Lee, G. Lee*, D.S. Yoon. Atomic Force Microscopy Analysis of EPPS-Driven Degradation and Reformation of β-Amyloid Aggregates. Journal of Alzheimer’s Disease Reports. 2(1), 41-49. 2018.
■ W. Kim, G. Lee#, M. Kim, J. Park, S. Jo, D.S. Yoon, Y.H. Park, J. Hong, J. Park. Al3+ Ion Sensing at Attomole Level via Surface-Potential Mapping of Gold Nanoparticle Complexes. Sensors and Actuators B-Chemical. 255 (Part 2), 2179-2186. 2018. 다수 언론에 본 연구성과가 보도됨.
■ S.W. Lee, W. Lee, Y. Hong, G. Lee*, D.S. Yoon. Recent Advances in Carbon Material-Based NO2 Gas Sensor. Sensors and Actuators B-Chemical. 255 (Part 2), 1788-1804. 2018.
■ W. Lee, J. Son, S. Kim, D. Yang, S. Choi, R.A. Watanabe, K.S. Hwang, S.W. Lee, G. Lee*, D.S. Yoon. Adhesive Leaf Created by a Corona Discharge. Scientific Reports. 8, 1737. 2018.
■ H. Lee, S.W. Lee, G. Lee#, W. Lee, K. Nam, J.H. Lee, K.S. Hwang, J. Yang, H. Lee, S. Kim, S.W. Lee, D.S. Yoon. Identifying DNA Mismatches at Single-Nucleotide Resolution by Probing Individual Surface Potentials of DNA-Capped Nanoparticles. Nanoscale. 10(2), 538-547. 2018. (Front Cover Paper).
■ W. Lee, H. Lee, Y. Choi, K.S. Hwang, S.W. Lee, G. Lee*, D.S. Yoon. Nanoelectrical Characterization of Amyloid-β42 Aggregates via Kelvin Probe Force Microscopy. Macromolecular Research. 25(12), 1187-1191. 2017.
■ J.W. Choi, H. Lee, G. Lee, Y.R. Kim, M.-J. Ahn, H.J. Park, K. Eom, T. Kwon. Blood Droplet-Based Cancer Diagnosis via Proteolytic Activity Measurement in Cancer Progression. Theranostics. 7(11), 2878-2887. 2017.
■ S. Choi, G. Lee#, I.S. Park, M. Son, W. Kim, H. Lee, S.-Y. Lee, S. Na, D.S. Yoon, R. Bashir, J. Park, S.W. Lee. Detection of Silver Ions Using Dielectrophoretic Tweezers-Based Force Spectroscopy. Analytical Chemistry. 88(22), 10867-10875. 2016. (Front Cover Paper).
■ W. Lee, I. Kim, S.W. Lee, H. Lee, G. Lee, S. Kim, S.W. Lee, D.S. Yoon. Quantifying Ascorbic Acid-Driven Inhibitory Effect on Amyloid Fibrillation. Macromolecular Research. 24(10), 868-873. 2016. (Front Cover Paper).
■ H. Lee, S.W. Lee, G. Lee, W. Lee, J.H. Lee, K.S. Hwang, J. Yang, S.W. Lee, D.S. Yoon. Kelvin Probe Force Microscopy of DNA-Capped Nanoparticles for Single-Nucleotide Polymorphism Detection. Nanoscale. 8(28), 13537-13544. 2016. (Front Cover Paper).
■ I.S. Park, T.J. Kwak, G. Lee, M. Son, J.W. Choi, S. Choi, K. Nam, S.-Y. Lee, W.-J. Chang, K. Eom, D.S. Yoon, S. Lee, R. Bashir, S.W. Lee. Biaxial Dielectrophoresis Force Spectroscopy: A Stoichiometric Approach for Examining Intermolecular Weak Binding Interactions. ACS Nano. 10(4), 4011-4019. 2016.
■ J.W. Hwang, J.-H. Choi, B. Choi, G. Lee, S.W. Lee, Y.-M. Koo, W.-J. Chang. Microfluidic Room Temperature Ionic Liquid Droplet Generation Depending on the Hydrophobicity and Interfacial Tension. Korean Journal of Chemical Engineering. 33(1), 57-62. 2016.
■ G. Lee, W. Lee, H. Lee, C.Y. Lee, K. Eom, T. Kwon. Self-Assembled Amyloid Fibrils with Controllable Conformational Heterogeneity. Scientific Reports. 5, 16220. 2015. 한국연구재단에 본 연구성과가 보도됨.
■ I.S. Park, J. Lee, G. Lee, K. Nam, T. Lee, W.-J. Chang, H. Kim, S.-Y. Lee, J. Seo, D.S. Yoon, S.W. Lee. Real-Time Analysis of Cellular Response to Small Molecule Drugs within a Microfluidic Dielectrophoresis Device. Analytical Chemistry. 87(12), 5914-5920. 2015. (Front Cover Paper).
■ D.H. Park, B.-K. Jung, H. Park, H. Lee, G. Lee, J. Park, U. Shin, J.H. Won, Y.J. Jo, J.W. Chang, S.W. Lee, D.S. Yoon, C.-W. Kim, J. Seo. Sound Packing DNA: Packing Open Circular DNA with Low-Intensity Ultrasound. Scientific Reports. 5, 9846. 2015.
■ M. Son, G. Lee#,*, J. Son, S. Choi, Y. Kim, S.-Y. Lee, Y.-R. Yoon, D.S. Yoon, S.W. Lee. Characterization of Anomalous Movements of Spherical Living Cells on a Silicon Dioxide Glassy Substrate. Biomicrofluidics. 9(1), 014102. 2015.
■ W. Lee, H. Jung, M. Son, H. Lee, T.J. Kwak, G. Lee, C.H. Kim, S.W. Lee, D.S. Yoon. Characterization of the Regrowth Behavior of Amyloid-Like Fragmented Fibrils Decomposed by Ultrasonic Treatment. RSC Advances. 4(100), 56561-56566. 2014.
■ T. Kwon, G. Lee, H. Choi, M.S. Strano, W.J. Kim. Highly Efficient Exfoliation of Individual Single-Walled Carbon Nanotubes by Biocompatible Phenoxylated Dextran. Nanoscale. 5(15), 6773-6778. 2013. (Front Cover Paper).
■ E. Kim, J. Yang, H.-O. Kim, Y. An, E.-K. Lim, G. Lee, T. Kwon, J.-H. Cheong, J.-S. Suh, Y.-M. Huh, S. Haam. Hyaluronic Acid Receptor-Targetable Imidazolized Nanovectors for Induction of Gastric Cancer Cell Death by RNA Interference. Biomaterials. 34(17), 4327-4338. 2013.
■ T. Kwon, J. Park, G. Lee, K. Nam, Y.-M. Huh, S.-W. Lee, J. Yang, C.Y. Lee, K. Eom. Carbon Nanotube-Patterned Surface-based Recognition of Carcinoembryonic Antigens in Tumor Cell for Cancer Diagnosis. Journal of Physical Chemistry Letters. 4(7),1126-1130. 2013.
■ K. Jang, K. Eom, G. Lee, J.-H. Han, S. Haam, J. Yang, E. Kim, W.-J. Kim, T. Kwon. Water-Stable Single-Walled Carbon Nanotubes Coated by Pyrenyl Polyethylene Glycol for Fluorescence Imaging and Photothermal Therapy. BioChip Journal. 6(4), 396-403. 2012.
■ G. Lee, H. Lee, K. Nam, J.-H. Han, J. Yang, S.W. Lee, D.S. Yoon, K. Eom, T. Kwon. Nanomechanical Characterization of Chemical Interaction between Gold Nanoparticles and Chemical Functional Groups. Nanoscale Research Letters. 7(1), 608. 2012.
■ K. Nam, K. Eom, J. Yang, J. Park, G. Lee, K. Jang, H. Lee, S.W. Lee, D.S. Yoon, C.Y. Lee, T. Kwon. Aptamer-Functionalized Nano-Pattern based on Carbon Nanotube for Sensitive, Selective Protein Detection. Journal of Materials Chemistry. 22(44), 23348-23356. 2012. (Front Cover Paper).
■ G. Lee, W. Lee, H. Lee, S.W. Lee, D.S. Yoon, K. Eom, T. Kwon. Mapping the Surface Charge Distribution of Amyloid Fibril. Applied Physics Letters. 101(4), 043703. 2012.
■ G. Lee, K. Eom, J. Park, J. Yang, S. Haam, Y.-M. Huh, J.K. Ryu, N.H. Kim, J.I. Yook, S.W. Lee, D.S. Yoon, T. Kwon. Real-Time Quantitative Monitoring of Specific Peptide Cleavage by a Proteinase for Cancer Diagnosis. Angewandte Chemie International Edition. 51(24), 5837-5841. 2012. (Inside Back Cover Paper) (highlighted in BRIC - link). 연합뉴스, KBS 등 다수 언론에 본 연구성과가 보도됨.
■ G. Lee, I. Park, K. Kwon, T. Kwon, J. Seo, W.J. Chang, H. Nam, G.S. Cha, M.H. Choi, D.S. Yoon, S.W. Lee. Electrochemical Detection of High-Sensitivity CRP inside a Microfluidic Device by Numerical and Experimental Studies. Biomedical Microdevices. 14(2), 375-384. 2012.
■ K. Eom, H. Jung, G. Lee, J. Park, K. Nam, S.W. Lee, D.S. Yoon, J. Yang, T. Kwon. Nanomechanical Actuation driven by Light-Induced DNA Fuel. Chemical Communications. 48(7), 955-957. 2012. (Front Cover Paper).
■ J. Park, J. Yang, G. Lee, C.Y. Lee, S. Na, S.W. Lee, S. Haam, Y.-M. Huh, D.S. Yoon, K. Eom, T. Kwon. Single-Molecule Recognition of Biomolecular Interaction via Kelvin Probe Force Microscopy. ACS Nano. 5(9), 6981-6990. 2011.
■ K. Nam, G. Lee#, H. Jung, J. Park, C.H. Kim, J. Seo, D.S. Yoon, S.W. Lee, T. Kwon. Single-Step Electropolymerization Patterning of a Polypyrrole Nanowire by Ultra-Short Pulses via An AFM Cantilever. Nanotechnology. 22(22), 225303. 2011.
■ G. Lee, H. Jung, J. Son, K. Nam, T. Kwon, G. Lim, Y.H. Kim, J. Seo, S.W. Lee, D.S. Yoon. Experimental and Numerical Study of Electrochemical Nanomachining Using an AFM Cantilever Tip. Nanotechnology. 21(18), 185301. 2010.

Book

■ D. Lee, I. Kim, S.W. Lee, G. Lee*, D.S. Yoon. Chapter #. Technical aspects and challenges of the paper-based colorimetric assay. Paper-Based Medical Diagnostic Devices (Springer Nature, UK) (Date: n/a, 2019) (DOI: n/a) (ISBN n/a).
■ I. Kim, G. Lee*, D.S. Yoon. Chapter 9. Application of Red Cell Membrane in Nanobiotechnology. Erythrocyte (InTech, London) (Date: Oct 23, 2019) (DOI: 10.5772/intechopen.84274) (ISBN 978-1-78984-210-4).
■ W. Lee, H. Lee, G. Lee, D.S. Yoon. Chapter 3. Advances in AFM imaging applications for characterizing the biophysical properties of amyloid fibrils. Exploring New Findings on Amyloidosis (InTech, London) (Date: Aug 24, 2016) (DOI: 10.5772/63316) (ISBN 978-953-51-2619-5). *Most Downloaded Chapter (>1,000 times to date).

고려대학교 생명정보공학과

■ 2020년 가을: 생화학2, 조직생명공학
■ 2020년 봄 : 생화학1, 나노생물공학
■ 2019년 겨울: 조직생명공학
■ 2019년 가을: 조직생명공학, 나노생물공학

고려대학교 바이오의공학부

■ 2019년 가을: 전자회로
■ 2019년 봄 : 생화학, 디지털시스템
■ 2018년 가을: 전자회로
■ 2018년 봄 : 생화학
■ 2017년 가을: 세포생물학, 면역학

Reasercher

■ 노석범 (학부연구생)
■ 이태하 (학부연구생)